Anti-Alpha B Crystallin antibody [CPTC-CRYAB-1] (ab268037)
Key features and details
- Mouse monoclonal [CPTC-CRYAB-1] to Alpha B Crystallin
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2c
Overview
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Product name
Anti-Alpha B Crystallin antibody [CPTC-CRYAB-1]
See all Alpha B Crystallin primary antibodies -
Description
Mouse monoclonal [CPTC-CRYAB-1] to Alpha B Crystallin -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Alpha B Crystallin aa 1-175.
Database link: P02511 -
Positive control
- WB: HeLa cel lysate. IHC-P: Human breast tissue; rat heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
CPTC-CRYAB-1 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
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Notes |
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Protein Array
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
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Target
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Function
May contribute to the transparency and refractive index of the lens. -
Tissue specificity
Lens as well as other tissues. -
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 1410 Human
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
- SwissProt: P02511 Human
- SwissProt: P23928 Rat
- Unigene: 53454 Human
- Unigene: 98208 Rat
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Alternative names
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
Images
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Formalin-fixed, paraffin-embedded Rat Heart Muscle tissue stained for Alpha B Crystallin using ab268037 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.
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Anti-Alpha B Crystallin antibody [CPTC-CRYAB-1] (ab268037) at 2 µg/ml + HeLa cell lysate
Predicted band size: 20 kDa
Western blot HeLa cell lysate using Crystallin Alpha B Mouse Monoclonal Antibody (CPTC-CYRAB-1). -
Formalin-fixed, paraffin-embedded human breast tissue stained for Alpha B Crystallin using ab268037 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.
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Protein Array containing more than 19,000 full-length human proteins using ab268037. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab268037 has not yet been referenced specifically in any publications.