Anti-nkx6.1 antibody [NKX61/2561] (ab268088)
Key features and details
- Mouse monoclonal [NKX61/2561] to nkx6.1
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2c
Overview
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Product name
Anti-nkx6.1 antibody [NKX61/2561]
See all nkx6.1 primary antibodies -
Description
Mouse monoclonal [NKX61/2561] to nkx6.1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human nkx6.1 aa 1-367. The exact sequence is proprietary.
Database link: P78426 -
Positive control
- IHC-P: Human pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
NKX61/2561 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Protein Array |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
Target
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Function
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals. -
Tissue specificity
Pancreatic beta cells. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Domain
The C-terminal domain contributes to sequence-specific DNA-binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4825 Human
- Omim: 602563 Human
- SwissProt: P78426 Human
- Unigene: 546270 Human
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Alternative names
- Homeobox protein NK-6 homolog A antibody
- Homeobox protein Nkx-6.1 antibody
- NK homeo box, family 6, member A antibody
see all
Images
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for nkx6.1 with ab268088 at 2 μg/ml in immunohistochemical analysis.
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Protein Array containing more than 19,000 full-length human proteins using ab268088. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for nkx6.1 with ab268088 at 2 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268088 has not yet been referenced specifically in any publications.