Recombinant Anti-Cyclin A1 antibody [EPR24373-146] (ab270940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24373-146] to Cyclin A1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cyclin A1 antibody [EPR24373-146]
See all Cyclin A1 primary antibodies -
Description
Rabbit monoclonal [EPR24373-146] to Cyclin A1 -
Host species
Rabbit -
Specificity
Not suitable for testing cell lines in WB.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis and His-tagged human cyclinA1 recombinant protein lysate IHC-P: Human testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24373-146 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/2000.
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Notes |
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WB
1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/2000. |
Target
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Function
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. -
Tissue specificity
Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8900 Human
- Omim: 604036 Human
- SwissProt: P78396 Human
- Unigene: 417050 Human
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Alternative names
- CCN A1 antibody
- CCNA 1 antibody
- Ccna1 antibody
see all
Images
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Anti-Cyclin A1 antibody [EPR24373-146] (ab270940) at 1/1000 dilution + Human testis tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 52 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
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All lanes : Anti-Cyclin A1 antibody [EPR24373-146] (ab270940) at 1/5000 dilution
Lane 1 : His-tagged human cyclinA1 recombinant protein (aa51-223)
Lane 2 : His-tagged human cyclinA2 recombinant protein (aa1-431)
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the pachytene spermatocytes of human testis (PMID: 14696091). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on human cardiac muscle (PMID: 9041194). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on human pancreas (PMID: 9041194). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab270940 has not yet been referenced specifically in any publications.