Biotin Anti-G-CSF antibody (ab271256)
Key features and details
- Biotin Rabbit polyclonal to G-CSF
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-G-CSF antibody
See all G-CSF primary antibodies -
Description
Biotin Rabbit polyclonal to G-CSF -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human G-CSF aa 1 to the C-terminus.
Database link: P09919 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 22 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 22 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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Western blot analysis of varying levels of Recombinant human G-CSF protein using ab271256 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant G-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Human hG-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271256. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab271256 has not yet been referenced specifically in any publications.