Biotin Anti-NGF antibody (ab271277)
Key features and details
- Biotin Rabbit polyclonal to NGF
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-NGF antibody
See all NGF primary antibodies -
Description
Biotin Rabbit polyclonal to NGF -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human NGF aa 100 to the C-terminus. Mature chain. E. coli derived.
Database link: P01138 -
Positive control
- WB: Recombinant human NGF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 26 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Human NGF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein. |
Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 26 kDa. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ELISA
Use a concentration of 0.25 - 1 µg/ml. Human NGF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein. |
Target
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Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
Involvement in disease
Neuropathy, hereditary sensory and autonomic, 5 -
Sequence similarities
Belongs to the NGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta nerve growth factor antibody
- Beta NGF antibody
- Beta-nerve growth factor antibody
see all
Images
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Lane 1: Marker.
Lane 2: Recombinant hNGF at 250ng/lane.
Lane 3: Recombinant hNGF at 125ng/lane.
Lane 4: Recombinant hNGF at 62.5ng/lane.
Lane 5: Recombinant hNGF at 31.25ng/lane.
Lane 6: Recombinant hNGF at 15.625ng/lane.
Lane 7: Recombinant hNGF at 7.8ng/lane.
Lane 8: Recombinant hNGF at 3.9ng/lane.
Lane 9: Recombinant hNGF at 1.95ng/lane.
Lane 10: Recombinant hNGF at 0.975ng/lane.
Lane 11: Recombinant hNGF at 0.4875ng/lane.
Lane 12: Recombinant hNGF at 0.24ng/lane. -
Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab271277 has not yet been referenced specifically in any publications.