Recombinant Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - BSA and Azide free (ab271892)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
Related conjugates and formulations
Overview
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Product name
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - BSA and Azide free
See all AMPK alpha 1+AMPK alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Molt-4 cell lysate. HEK-293 cell lysate. Fruit fly lysate. Rat brain lysate, Mouse heart lysate.
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General notes
ab271892 is the carrier-free version of ab133448.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.22 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5683 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Target
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- Omim: 602739 Human
see all
Images
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)
Lane 1 : 293 cell lysate
Lane 2 : Lysate of 293 cells treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilutionThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Untreated HEK-293 cell lysate
Lane 2 : HEK-293 cell lysate treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilutionBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution
Lane 1 : HEK-293 (human embryonic kidney) whole cell lysates
Lane 2 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
Lane 3 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (133448).
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133448).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271892 has not yet been referenced specifically in any publications.