Recombinant Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free (ab271932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16800] to Integrin alpha V - BSA and Azide free
- Suitable for: IP, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR16800] to Integrin alpha V - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
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General notes
ab271932 is the carrier-free version of ab179475.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16800 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 116 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 116 kDa. |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.
Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179475). -
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.
Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179475). -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.
Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179475). -
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).
Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179475). -
Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: A549 whole cell extract.
Lane 2: PBS instead of A549 whole cell extract.
Blocking/Dilution buffer: 5% NFDM/TBST.ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179475).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271932 has not yet been referenced specifically in any publications.