Recombinant Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free
See all Laminin alpha 5/LAMA5 primary antibodies -
Description
Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody reacts weakly with Laminin alpha 3
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
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General notes
ab271947 is the carrier-free version of ab184330.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18919 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 404 kDa (predicted molecular weight: 400 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 404 kDa (predicted molecular weight: 400 kDa). |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
Sequence similarities
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3911 Human
- Entrez Gene: 16776 Mouse
- Entrez Gene: 140433 Rat
- Omim: 601033 Human
- SwissProt: O15230 Human
- SwissProt: Q61001 Mouse
- Unigene: 473256 Human
- Unigene: 4339 Mouse
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Alternative names
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
Images
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All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 400 kDa
Observed band size: 404 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, sodium azide and BSA (ab184330).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271947 has not yet been referenced specifically in any publications.