Recombinant Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - BSA and Azide free
- Suitable for: IP, IHC-Fr, IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free
See all Prosurfactant Protein C primary antibodies -
Description
Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
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General notes
ab271991 is the carrier-free version of ab211326.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19839 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. -
Involvement in disease
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
Sequence similarities
Contains 1 BRICHOS domain. -
Cellular localization
Secreted > extracellular space > surface film. - Information by UniProt
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Database links
- Entrez Gene: 20389 Mouse
- SwissProt: P21841 Mouse
- Unigene: 24040 Mouse
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Alternative names
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
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SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Mouse lung lysate, 10ug (Input).
Lane 2: ab211326 IP in mouse lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The banding pattern represents pro- and mature forms of SFTPC.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271991 has not yet been referenced specifically in any publications.