Recombinant Anti-PAP antibody [SP313] - BSA and Azide free (ab272004)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP313] to PAP - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PAP antibody [SP313] - BSA and Azide free
See all PAP primary antibodies -
Description
Rabbit monoclonal [SP313] to PAP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: LNCaP cells. IHC-P: Human prostate adenocarcinoma tissue.
-
General notes
ab272004 is the carrier-free version of ab224802.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP313 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Sequence similarities
Belongs to the histidine acid phosphatase family. -
Cellular localization
Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles. - Information by UniProt
-
Database links
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
-
Alternative names
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
see all
Images
-
Flow cytometric analysis of LNCaP (human prostate cancer cell line) cells labeling PAP with ab224802 at 1/100 dilution (green) compared with a rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224802).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab272004 has not yet been referenced specifically in any publications.