Recombinant Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR072G] to HAUSP / USP7 - BSA free
- Suitable for: IP, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-HAUSP / USP7 antibody [BLR072G] - BSA free
See all HAUSP / USP7 primary antibodies -
Description
Rabbit monoclonal [BLR072G] to HAUSP / USP7 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human HAUSP/ USP7 aa 1-50. The exact sequence is proprietary. NP_003461.2
Database link: Q93009 -
Positive control
- IHC-P: Human prostate carcinoma tissue. Mouse renal cell carcinoma tissue. ICC; OVCAR-4 cells. IP: HEK-293T whole cell lysate. WB: RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, TCMK-1
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General notes
ab272079 is the BSA-free version of ab264422.
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR072G -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 128 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 128 kDa. |
Target
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Function
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222). -
Tissue specificity
Widely expressed. Overexpressed in prostate cancer. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 MATH domain.
Contains 1 USP domain. -
Domain
The C-terminus plays a role in its oligomerization. -
Post-translational
modificationsIsoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869. -
Cellular localization
Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 7874 Human
- Entrez Gene: 252870 Mouse
- Omim: 602519 Human
- SwissProt: Q93009 Human
- SwissProt: Q6A4J8 Mouse
- Unigene: 386939 Human
- Unigene: 295330 Mouse
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Alternative names
- Deubiquitinating enzyme 7 antibody
- HAUSP antibody
- Herpes virus associated ubiquitin specific protease antibody
see all
Images
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All lanes : Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079) at 1/1000 dilution
Lane 1 : RKO whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 8 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 9 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 10 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 128 kDa
Exposure time: 3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
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Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 (Human ovarian cancer cell line) cells labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
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HAUSP / USP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264422 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264422 at 1/1000 dilution
Lane 1: ab264422 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272079 has not yet been referenced specifically in any publications.