Anti-VKORC1L1 antibody (ab272657)
Key features and details
- Rabbit polyclonal to VKORC1L1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VKORC1L1 antibody -
Description
Rabbit polyclonal to VKORC1L1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human VKORC1L1 aa 150-250.
Database link: Q8N0U8 -
Positive control
- WB: NTERA-2 cells. IHC-P: Human stomach tissue. IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Belongs to the VKOR family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 154807 Human
- Entrez Gene: 69568 Mouse
- Entrez Gene: 399684 Rat
- Omim: 608838 Human
- SwissProt: Q8N0U8 Human
- SwissProt: Q6TEK5 Mouse
- SwissProt: Q6TEK3 Rat
- Unigene: 427232 Human
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Alternative names
- DKFZp762H0113 antibody
- Vitamin K epoxide reductase complex subunit 1 like 1 antibody
- Vitamin K epoxide reductase complex subunit 1-like protein 1 antibody
see all
Images
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Lane 2 : Anti-VKORC1L1 antibody (ab272657) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : NTERA-2 cells (Human malignant pluripotent embryonic carcinoma cell line) -
Paraffin-embedded formalin-fixed human stomach tissue stained for VKORC1L1 using ab272657 at 1/50 dilution in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeablized A549 cells (human lung carcinoma cell line) labeling VKORC1L1 (green) using ab272657 at 2 ug/ml in ICC/IF analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272657 has not yet been referenced specifically in any publications.