AMP Assay Kit (Colorimetric) (ab273275)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
AMP Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples.
The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K229 AMP Colorimetric Assay Kit. K229-100 is the same size as the 100 test size of ab273275.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMP 1 x 200µl Assay Buffer XV 1 x 25ml AMP Substrate Mix 1 vial Developer V 1 vial Development Enzyme Mix III 1 x 200µl OxiRed Probe 1 x 200µl
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273275 has not yet been referenced specifically in any publications.