Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Cell Lysate, Other biological fluids, Tissue Lysate
Overview
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Product name
Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332) utilizes LPC-specific enzymes to generate an intermediate that then reacts with a probe, yielding a signal that can be quantified either colorimetrically or fluorometrically, and is proportional to the amount of LPC present in the sample.
When used as described, the assay is capable of detecting as little as 10 pmole of Lysophosphatidylcholine.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K735 Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric). K735-100 is the same size as the 100 test size of ab273332.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 100 tests Assay Buffer LXI 1 x 25ml 1 x 25ml LPC Developer Mix 1 vial 1 vial LPC Enzyme Mix Lyophilized 1 vial 1 vial LPC Standard 1 vial 1 vial Lysis Buffer II 1 x 1ml 2 x 1ml OxiRed Probe 1 x 200µl 1 x 200µl
Images
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Example data.
LPC Standard curve, fluorometric.
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Example data.
LPC Standard curve, colorimetric.
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Example data.
Determination of total LPC concentration in pooled human serum. Sample was analyzed according to the kit protocol. Values were determined with fluorometric measurements and are mean ± standard deviation of at least three independent determinations.
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Example data.
Specificity of the assay: PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, LPC = lysophosphatidylcholine. 1 nmole of each was tested in the fluorometric assay.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273332 has not yet been referenced specifically in any publications.