PE Anti-14-3-3 sigma/SFN antibody [02] (ab275638)
Key features and details
- PE Mouse monoclonal [02] to 14-3-3 sigma/SFN
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-14-3-3 sigma/SFN antibody [02]
See all 14-3-3 sigma/SFN primary antibodies -
Description
PE Mouse monoclonal [02] to 14-3-3 sigma/SFN -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human 14-3-3 sigma/SFN aa 1 to the C-terminus. Two additional amino acids (Gly and Pro) at the N-terminal.
Database link: P31947 -
Positive control
- Flow Cyt (Intra): HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
02 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
10 µl/Test |
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. 10 µl/Test |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
Tissue specificity
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
Cellular localization
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 2810 Human
- Omim: 601290 Human
- SwissProt: P31947 Human
- Unigene: 523718 Human
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Alternative names
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
Images
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Intracellular flow cytometric analysis ofHeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SFN with ab275638 at 10 µl/Test (Black) compared with isotype control (Grey). The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab275638 has not yet been referenced specifically in any publications.