Anti-SMNDC1 antibody [PCRP-SMNDC1-1A9] (ab277099)
Key features and details
- Mouse monoclonal [PCRP-SMNDC1-1A9] to SMNDC1
- Suitable for: Protein Array, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-SMNDC1 antibody [PCRP-SMNDC1-1A9]
See all SMNDC1 primary antibodies -
Description
Mouse monoclonal [PCRP-SMNDC1-1A9] to SMNDC1 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SMNDC1 aa 1-238.
Database link: O75940 -
Positive control
- Flow cyt: HeLa cells. ICC: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99.9% PBS -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate -
Clonality
Monoclonal -
Clone number
PCRP-SMNDC1-1A9 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Protein Array |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use 1-2µg for 106 cells.
|
|
ICC |
Use a concentration of 1 - 2 µg/ml.
|
Notes |
---|
Protein Array
Use at an assay dependent concentration. |
Flow Cyt
Use 1-2µg for 106 cells. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
-
Function
Necessary for spliceosome assembly. Overexpression causes apoptosis. -
Tissue specificity
Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas. -
Sequence similarities
Belongs to the SMN family.
Contains 1 Tudor domain. -
Cellular localization
Nucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies. - Information by UniProt
-
Database links
- Entrez Gene: 10285 Human
- Omim: 603519 Human
- SwissProt: O75940 Human
- Unigene: 632093 Human
-
Alternative names
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
see all
Images
-
Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SMNDC1 with ab277099 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
-
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SMNDC1 using ab277099 at 2 µg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
-
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277099.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab277099 has not yet been referenced specifically in any publications.