COX2 Inhibitor Screening Kit (Fluorometric) (ab283401)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
Overview
-
Product name
COX2 Inhibitor Screening Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay duration
Multiple steps standard assay -
Product overview
COX2 Inhibitor Screening Kit (Fluorometric) (ab283401) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of COX-2 inhibitors.
Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase, , is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-2 is not expressed under normal conditions in most cells, but elevated levels are found during inflammation.
This assay is based on the fluorometric detection of Prostaglandin G2, the intermediate product generated by the COX enzyme.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K547 COX-2 Inhibitor Screening Kit (Fluorometric). K547-100 is the same size as the 100 test size of ab283401.
-
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Arachidonic Acid 1 vial Celecoxib (COX-2 Inhibitor) 1 x 100µl COX Assay Buffer 1 x 25ml COX Cofactor 1 x 20µl Human Recombinant COX-2 1 vial NaOH Solution 1 x 500µl OxiRed Probe 1 x 200µl -
Research areas
-
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Alternative names
- COX 2
- COX-2
- Cyclooxygenase 2
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab283401 has not yet been referenced specifically in any publications.