Recombinant Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (ab283512)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24403-17] to CD204 - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD204 antibody [EPR24403-17] - BSA and Azide free
See all CD204 primary antibodies -
Description
Rabbit monoclonal [EPR24403-17] to CD204 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human liver and Human spleen tissue lysate; THP-1 treated with 80nM PMA for 72 hours whole cell lysate. IHC-P: Human liver, Human lung, Human spleen, Human gastric carcinoma and Human hepatocellular carcinoma tissue. ICC/IF: THP-1 cells treated with PMA Flow Cyt: THP-1 cells treated with PMA IP: THP-1 cells treated with PMA
-
General notes
ab283512 is the carrier-free version of ab271070.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24403-17 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
Flow Cyt
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. -
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4481 Human
- Omim: 153622 Human
- SwissProt: P21757 Human
- Unigene: 147635 Human
-
Alternative names
- CD204 antibody
- CD204 antigen antibody
- CD24 antibody
see all
Images
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells treated with 80nM phorbol 12-myristate 13-acetate (PMA) for 72 hours (Right) / Untreated control (Left), labelling CD204 with ab271070 at 1/50 dilution (1ug). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Kupffer cells in human liver (PMID: 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on macrophages in human lung. The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in human gastric carcinoma (PMID: 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in human hepatocellular carcinoma (PMID: 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling CD204 with ab271070 at 1/50 (10.32 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (80 nM) for 72 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
-
All lanes : Anti-CD204 antibody [EPR24403-17] (ab271070) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 4 : THP-1 treated with 80nM PMA for 72 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using ab271070, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30991038).
Exposure time: Lanes 1-2: 26 seconds; Lanes 3-4: 8 seconds.
-
This data was developed using ab271070, the same antibody clone in a different buffer formulation.
CD204 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte), treated with 80nM PMA for 72 hours, whole cell lysate with ab271070 at 1/30 dilution (2 ug in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab271070 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: THP-1(human monocytic leukemia monocyte) treated with 80nM PMA for 72 hours whole cell lysate 20 ug
Lane 2: ab271070 IP in THP-1 treated with 80nM PMA for 72 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271070 in THP-1 treated with 80nM PMA for 72 hours whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab283512 has not yet been referenced specifically in any publications.