Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - BSA and Azide free (ab283943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: ELISA, WB, Dot blot
Related conjugates and formulations
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - BSA and Azide free
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Our data (not shown) suggests this RabMAb is interacting with the non-receptor binding domain of SARS-CoV-2 Spike Glycoprotein S1 with a higher affinity (~ 71x ) than the receptor binding domain of SARS-CoV-2 Spike Glycoprotein S1.
This antibody cross-reacts with the SARS-CoV-2 Omicron variant spike protein.
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Tested applications
Suitable for: ELISA, WB, Dot blotmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SARS-CoV-2 ORF8. ELISA: SARS-CoV2 ORF8.
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General notes
ab283943 is the carrier-free version of ab283942
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24852-116 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 4e-005 - 0.1 µg/ml.
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WB |
1/1000.
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Dot blot |
1/1000.
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Notes |
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ELISA
Use a concentration of 4e-005 - 0.1 µg/ml. |
WB
1/1000. |
Dot blot
1/1000. |
Images
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] (ab283942) at 1/1000 dilution
All lanes :Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Lysates/proteins at 0.2 µg per lane.This data was developed using ab283942, the same antibody clone in a different buffer formulation.
Secondaries
Lane 1: Red – loading control Mouse anti-6x His tag antibody (ab18184) observed at 135 kDa
Lanes 2: Green – ab283942 observed at 135 kDa
ab283942 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T)
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] (ab283942) at 1/1000 dilution
Lanes 1 & 3 : Expi293 cells transfected with SARS-CoV2 3xFlag Spike Protein
Lanes 2 & 4 : Expi293 cells transfected with SARS-CoV1 3xFlag Spike Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution. at 1/20000 dilutionThis data was developed using ab283942, the same antibody clone in a different buffer formulation.
Observed band size: 200 kDa
Calculated: 140kDa
Secondaries
Lane 1 & 2: Red – loading control Goat anti-DDDDK tag antibody (ab95045, Binds to FLAG tag sequence) observed at 200 kDa
Lanes 3 & 4: Green – ab283942 observed at 200 kDa
ab283942 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T)
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This data was developed using ab283942, the same antibody clone in a different buffer formulation.
Indirect ELISA showing primary antibody ab283942 binding to the antigen ab272105 (recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera)). Plates were coated with recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105) and recombinant human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab283942 was assessed in a serial dilution range 0.04- 100 ng/mL (a 3-fold serial dilution).
Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab283943 has not yet been referenced specifically in any publications.