Human Immunoglobulin J Chain ELISA Kit (ab283987)
Key features and details
- Sensitivity: 0.18 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Immunoglobulin J Chain ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 5.7% Inter-assay Sample n Mean SD CV% Overall 60 10.5% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Extracts, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.18 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Immunoglobulin J Chain ELISA Kit (ab283987) is designed for detection of Immunoglobulin J (IGJ) in human plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IGJ in approximately 5 hours. A polyclonal antibody specific for human IGJ has been pre-coated onto a 96-well microplate with removable strips. IGJ in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human IGJ, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Immunoglobulin J Chain Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Human Immunoglobulin J Chain Microplate 1 unit Immunoglobulin J Chain Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IGCJ
- IGJ_HUMAN
- Immunoglobulin J chain
see all -
Database links
- Entrez Gene: 3512 Human
- Omim: 147790 Human
- SwissProt: P01591 Human
- Unigene: 643431 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab283987 has not yet been referenced specifically in any publications.