Human PR3 Autoantibody ELISA Kit (ab284018)
Key features and details
- Sensitivity: 0.65 AU/ml
- Range: 1.875 AU/ml - 30 AU/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PR3 Autoantibody ELISA Kit
See all PR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 5.7% Inter-assay Sample n Mean SD CV% Overall 60 9.9% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.65 AU/ml -
Range
1.875 AU/ml - 30 AU/ml -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Proteinase 3 Autoantibody ELISA (ab284018) Kit is designed for the quantitative determination of autoimmune response (IgG) to a target antigen (PR3). The kit detects autoantibodies in plasma and serum samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures autoantibodies (anti-PR3 IgG) in approximately 4 hours. A PR3 antigen has been pre-coated onto a 96-well microplate with removable strips. An autoantibody specific for PR3 in standards and samples is sandwiched by the immobilized antigen and a biotinylated polyclonal antibody specific for human IgG, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Autoimmune Biotinylated Human IgG Antibody 1 vial Chromogen Substrate 1 vial Human PR3 Autoantibody Microplate 1 unit PR3 Autoantibody Standard 1 vial Sealing Tapes 3 units Stop Solution 1 vial -
Research areas
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- ACPA
- AGP 7
- AGP7
see all -
Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284018 has not yet been referenced specifically in any publications.