Recombinant Anti-SARS-CoV-2 nsp2 antibody [EPR24853-4] (ab284036)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24853-4] to SARS-CoV-2 nsp2
- Suitable for: ELISA, WB, ICC/IF, IHC-P, Flow Cyt (Intra)
Related conjugates and formulations
Overview
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Product name
Anti-SARS-CoV-2 nsp2 antibody [EPR24853-4]
See all SARS-CoV-2 nsp2 primary antibodies -
Description
Rabbit monoclonal [EPR24853-4] to SARS-CoV-2 nsp2 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Expi293 cells transfected with SARS-CoV2 NSP2 protein (His tagged), Recombinant SARS-CoV2 NSP2 protein (His tagged) Elisa: Recombinant SARS-COV-2 NSP2 protein (His-tag) IHC: HEK-293T transfected with SARS-CoV-2 NSP2 expression vector containing a his tag. ICC/IF: HEK-293T cells transfected with a SARS-CoV-2 nsp2 expression vector containing a myc tag. Flow: HEK-293T cells transfected with a SARS-CoV-2 nsp2 expression vector containing a myc tag.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR24853-4 -
Isotype
IgG
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 2e-005 - 1 µg/ml.
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WB | (1) |
1/1000. Detects a band of approximately 75 kDa.
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ICC/IF | (1) |
1/100.
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IHC-P |
1/100.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. |
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Flow Cyt (Intra) |
1/500.
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Notes |
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ELISA
Use a concentration of 2e-005 - 1 µg/ml. |
WB
1/1000. Detects a band of approximately 75 kDa. |
ICC/IF
1/100. |
IHC-P
1/100. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. |
Flow Cyt (Intra)
1/500. |
Images
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Flow cytometric analysis of 4% Paraformaldehyde fixed 90% methanol permeabilised HEK-293T (Human embryonic kidney epithelial) cells transfected with a SARS-CoV-2 nsp1 (Left) or SARS-CoV-2 nsp2 (Right) expression vector containing a myc tag labelling SARS-CoV-2 nsp2 with ab284036 at 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/5000 dilution was used as the secondary antibody.
ab284036 doesn’t cross-react with SARS-CoV-2 nsp1.
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Immunohistochemical analysis of Paraffin-embedded sections Human (A) HEK-293T transfected with SARS-CoV-2 NSP2 expression vector containing a his tag and (B) HEK-293T transfected empty vector containing a his tag tissue labelling SARS-CoV-2 nsp2 with ab284036 at 1/100 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). HEK-293T transfected with SARS-CoV-2 NSP2 expression vector containing a his tag staining on Human (A) HEK-293T transfected with SARS-CoV-2 NSP2 expression vector containing a his tag, (B) HEK-293T transfected empty vector containing a his tag is observed. Positive staining on HEK-293T transfected with SARS-CoV-2 NSP2 expression vector containing a his tag (Image A); No staining on HEK-293T transfected empty vector containing a his tag (Image B). The section was incubated with ab284036 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval using Bond® Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
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Immunohistochemical analysis of Paraffin-embedded sections Rat Liver tissue labelling SARS-CoV-2 nsp2 with ab284036 at 1/100 dilution (5 µg/ml), followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection) at Ready to use dilution.
Negative control: no staining on rat liver. The section was incubated with ab284036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval using Bond® Epitope Retrieval Solution 2 (pH 9.0) for 30 minutes.
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Immunohistochemical analysis of Paraffin-embedded sections Mouse Liver tissue labelling SARS-CoV-2 nsp2 with ab284036 at 1/100 dilution (5 µg/ml), followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).
Negative control: no staining on mouse liver. The section was incubated with ab284036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Heat mediated antigen retrieval using Bond® Epitope Retrieval Solution 2 (pH 9.0) for 30 minutes.
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Immunohistochemical analysis of Paraffin-embedded sections Human Liver tissue labelling SARS-CoV-2 nsp2 with ab284036 at 1/100 dilution (5 µg/ml), followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).
Negative control: no staining on human liver. The section was incubated with ab284036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Heat mediated antigen retrieval using Bond® Epitope Retrieval Solution 2 (pH 9.0) for 30 minutes.
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Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% TritonX-100 permeabilised HEK-293T (Human embryonic kidney 293) cells labelling Anti-SARS-CoV-2 nsp2 with ab284036 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV-2 nsp2 expression vector containing a myc tag, and no staining in HEK-293T cells transfected with a SARS-CoV-2 nsp1 expression vector containing a myc tag is observed. Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain Myc-Tag at 1/100 dilution (Red). The Nuclear counterstain was DAPI.
Secondary antibody only control: AlexaFluor®488 Goat anti-Rabbit (ab150081) at 1/1000 dilution.
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All lanes : Anti-SARS-CoV-2 nsp2 antibody [EPR24853-4] (ab284036) at 1/1000 dilution
All lanes : Recombinant SARS-CoV2 NSP2 protein (His tagged)
Lysates/proteins at 0.2 µg per lane.
Secondary
Lane 1 : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))
Lane 2 : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))
Observed band size: 75 kDa why is the actual band size different from the predicted?Observed band size: 75 kDa
Lanes 1: Red – loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 75 kDa.
Lanes 2: Green – ab284036 observed at 75 kDa
ab284036 was shown to bind specifically to SARS-CoV-2 NSP2 protein in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Blocking buffer: 5% milk in TBS-0.1% Tween® 20 (TBS-T)
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All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
All lanes : Expi293 cells transfected with SARS-CoV2 NSP2 protein (His tagged)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution
Observed band size: 75 kDa why is the actual band size different from the predicted?Observed band size: 75 kDa
Lanes 1: Red – loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 75 kDa.
Lanes 2: Green – ab284036 observed at 75 kDa
ab284036 was shown to bind specifically to SARS-CoV-2 NSP2 protein in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Blocking buffer: 5% milk in TBS-0.1% Tween® 20 (TBS-T)
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Indirect ELISA showing primary antibody ab284036 binding to recombinant SARS-COV-2 NSP2 protein (His-tag). Plates were coated with recombinant SARS-COV-2 NSP2 protein (His-tag) at 1000 ng/ml. Binding of ab284036 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).
Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284036 has not yet been referenced specifically in any publications.