1,5-Anhydroglucitol Assay Kit (Colorimetric) (ab284531)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Plasma, Saliva, Serum
Overview
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Product name
1,5-Anhydroglucitol Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Saliva, Serum, Plasma -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
1,5-Anhydroglucitol Assay Kit (Colorimetric) (ab284531) (K2029) is a high-throughput adaptable microplate-based assay that enables rapid quantification of 1,5-AG levels in biological fluids. The assay consists of two steps: in the first step, the sample is pretreated to remove endogenous glucose that may interfere with the 1,5-AG detection and 1,5-AG is converted to 1,5-AG-6-phosphate. In the second step, 1,5-AG-6- phosphate is enzymatically metabolized, generating NADPH, which subsequently reacts with a colorless probe to form a strongly colored product measured by absorbance at OD = 460 nm. The absorbance is proportional to the concentration of 1,5-AG present in the samples.
The assay is not affected by galactose, fructose or other physiologically common sugars and has a limit of detection of 0.11 nmoles of 1,5- AG per well, with a reliable linear range from 0.2 – 5 nmole per well (corresponding to a 20-500 μM concentration in a 10 μl sample volume).
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2029 1,5-Anhydroglucitol Assay Kit (Colorimetric). K2029-100 is the same size as the 100 test size of ab284531.
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Tested applications
Suitable for: Enzyme activity assaymore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 1,5-AG Standard 1 vial 96-Well Half Area Plate 1 unit Detection Buffer 1 x 10ml Detection Enzyme Mix 1 vial NADP Detection Probe 1 vial Pretreatment Cofactor Mix 1 vial Pretreatment Enzyme Mix 1 vial Sample Pretreatment Buffer 1 x 10ml
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Enzyme activity assay |
Use at an assay dependent concentration.
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Notes |
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Enzyme activity assay
Use at an assay dependent concentration. |
Images
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1,5-Anhydroglucitol (1,5-AG) Standard Curve. 1,5-AG concentration is directly proportional to the absorbance measured at 460 nm. For demonstrative purposes only.
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Estimation of 1,5-AG in two different single-donor “off-the-clot” human serum samples (10 μl), each spiked with 50 μM 1,5- AG. Mean 1,5-AG concentrations detected in the samples were 149.4 ± 14.2 μM and 66.1 ± 7.2 μM, respectively (mean spike recovery rates for the two samples were 107.9% and 101.6%, respectively). Data are mean ± SD of 4-5 replicates, assayed according to the kit protocol. For demonstrative purposes only.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284531 has not yet been referenced specifically in any publications.