Human SEMA4C ELISA Kit (ab284623)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23.5 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SEMA4C ELISA Kit
See all SEMA4C/SEMAI kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3.7% Inter-assay Sample n Mean SD CV% Plasma 3 9.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
23.5 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 93% - 97% Serum 87 84% - 94% Hep Plasma 94 92% - 99% EDTA Plasma 85 80% - 92% Cit plasma 81 80% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SEMA4C ELISA kit (ab284623) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SEMA4C protein in human serum, plasma, cell culture supernatant and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SEMA4C with 23.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SEMA4C Capture Antibody 1 x 600µl 10X Human SEMA4C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human SEMA4C Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. - Information by UniProt
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Alternative names
- KIAA1739
- M-SEMA-F
- S4c
see all -
Database links
- Entrez Gene: 54910 Human
- Omim: 604462 Human
- SwissProt: Q9C0C4 Human
- Unigene: 516220 Human
Images
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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Example of human SEMA4C standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human SEMA4C standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native SEMA4C was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma-EDTA, plasma-Heparin, and MCF-7 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentration of native SEMA4C was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL MCF-7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean SEMA4C concentration was determined to be 301.3 pg/mL with a range of 126.8 – 570.3 pg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284623 has not yet been referenced specifically in any publications.