Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free (ab284680)
Key features and details
- Rabbit monoclonal [EP734Y] to alpha Adducin (phospho S726) - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
-
Product name
Anti-alpha Adducin (phospho S726) antibody [EP734Y] - BSA and Azide free
See all alpha Adducin primary antibodies -
Description
Rabbit monoclonal [EP734Y] to alpha Adducin (phospho S726) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa cell lysate. IHC-P: Human lung tissue. ICC/IF: HeLa cells.
-
General notes
ab284680 is the carrier-free version of ab40760
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP734Y -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 118 Human
- Omim: 102680 Human
- SwissProt: P35611 Human
- Unigene: 183706 Human
-
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
-
All lanes : Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking and diluting buffer: 5% NFDM/TBST
This data was developed using ab40760, the same antibody clone in a different buffer formulation.
-
Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab40760, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab284680 has not yet been referenced specifically in any publications.