Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782)
Key features and details
- Rabbit monoclonal [EP2827] to HOXB5 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-HOXB5 antibody [EP2827] - BSA and Azide free
See all HOXB5 primary antibodies -
Description
Rabbit monoclonal [EP2827] to HOXB5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal lung and Human fetal kidney lysates Flow Cyt (Intra): U-87 MG
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General notes
ab284782 is the carrier-free version of ab109375
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2827 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Tissue specificity
Spinal cord. -
Sequence similarities
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Embryo. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3215 Human
- Omim: 142960 Human
- SwissProt: P09067 Human
- Unigene: 654456 Human
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Alternative names
- HGNC:5116 antibody
- HHO.C10 antibody
- Homeo box 2A antibody
see all
Images
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This data was developed using ab109375, the same antibody clone in a different buffer formulation.
ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.
Rabbit monoclonal IgG was used as an Isotype control (Green)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab284782 has not yet been referenced specifically in any publications.