Anti-M Cadherin antibody [EP5756] - BSA and Azide free (ab284800)
Key features and details
- Rabbit monoclonal [EP5756] to M Cadherin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-M Cadherin antibody [EP5756] - BSA and Azide free
See all M Cadherin primary antibodies -
Description
Rabbit monoclonal [EP5756] to M Cadherin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates; Mouse brain, heart, kidney and spleen tissues; Rat brain, kidney and spleen tissues.
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General notes
ab284800 is the carrier-free version of ab129078
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5756 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
Tissue specificity
Expressed in the brain and cerebellum. -
Involvement in disease
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- Omim: 114019 Human
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
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Alternative names
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
Images
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Rat Brain Tissue
Lane 2 : Rat Heart Tissue
Lane 3 : Rat Kidney Tissue
Lane 4 : Rat Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab129078, the same antibody clone in a different buffer formulation.
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml
Lane 1 : Mouse Brain Tissue
Lane 2 : Mouse Heart Tissue
Lane 3 : Mouse Kidney Tissue
Lane 4 : Mouse Spleen Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab129078, the same antibody clone in a different buffer formulation.
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Human cerebellum cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal brain cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP
Predicted band size: 89 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab129078, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab284800 has not yet been referenced specifically in any publications.