DYKDDDDK-Tag Protein ELISA Kit (ab285234)
Key features and details
- Sensitivity: 5 nM
- Range: 5 nM - 500 nM
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
Overview
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Product name
DYKDDDDK-Tag Protein ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Competitive -
Sensitivity
>= 5 nM -
Range
5 nM - 500 nM -
Assay time
1h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Species independent -
Product overview
This DYKDDDDK-Tag Protein ELISA Kit (ab285234, E4700) is based on the competitive ELISA principle and it is an easy, fast and sensitive method to detect the expressed DYKDDDDK-tag proteins. This detection kit offers ready-to-use reagents, and can detect as low as 5 nM of DYKDDDDK-tag protein in approximately 1.5 hrs.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4700 DYKDDDDK-Tag Protein ELISA Kit. E4700-100 is the same size as the 96 test size of ab285234.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 10X Wash Buffer II 1 x 50ml DYKDDDDK Antibody 1 x 7ml Conjugate Buffer III 1 x 7.5ml DYKDDDDK Standard 1 vial DYKDDDDK Microplate 1 unit Goat Anti-Rabbit HRP Conjugate I 1 x 25µl Microplate Sealing Film 4 units Stop Solution VIII 1 x 10ml TMB Substrate I 1 x 10ml -
Research areas
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag
- DDDK
- ddk
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285234 has not yet been referenced specifically in any publications.