His-tag Protein ELISA kit (ab285248)
Key features and details
- Sensitivity: 20 ng/ml
- Range: 25.6 ng/ml - 3200 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
His-tag Protein ELISA kit -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Competitive -
Sensitivity
20 ng/ml -
Range
25.6 ng/ml - 3200 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research.
His-tag Protein ELISA Kit (ab285248, E4550) is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells.
Detection method- Absorbance (450 nm)
Species reactivity- Universal -
Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 10X Wash Buffer II 1 x 50ml Antibody Diluent 1 x 7ml His-Tag Antibody 1 x 20µl Conjugate Buffer I 1 x 7.5ml His-Tag Coated Plate 1 unit Goat Anti-Rabbit HRP Conjugate I 1 x 25µl Microplate Sealing Film 4 units Sample Diluent IV 1 x 20ml His-Tag Standard 2 vials Stop Solution VIII 1 x 10ml TMB Substrate I 1 x 10ml
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab285248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285248 has not yet been referenced specifically in any publications.