Human CD27 ELISA Kit (ab285272)
Key features and details
- Sensitivity: 0.094 ng/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD27 ELISA Kit
See all CD27 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma, Other biological fluids, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.094 ng/ml -
Range
0.156 ng/ml - 10 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD27 ELISA Kit (ab285272, E4547) is used for quantitative measurement of CD27 in serum, plasma, tissue homogenates and other biological fluids.
CD27 is the receptor for CD70/CD27L. It plays a role in survival of activated T-cells and in apoptosis through association with SIVA1. The CD27 ELISA kit ab285272 is a sandwich ELISA assay for the quantitative measurement of CD27 in human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CD27 captured from the samples.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin/Detection antibody (Concentrated) 1 x 120µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 2 vials Micro ELISA Plate 1 unit Plate Sealer 5 units SABC dilution buffer 1 x 10ml Sample / Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD 27
- CD27
- CD27 antigen
see all -
Database links
- Entrez Gene: 939 Human
- Omim: 186711 Human
- SwissProt: P26842 Human
- Unigene: 355307 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285272 has not yet been referenced specifically in any publications.