Salmonella Typhi IgM ELISA Kit (ab285279)
Key features and details
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Salmonella Typhi IgM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 1 16 0.079 = 4.51% Serum 2 16 0.068 = 5.57% Serum 3 16 0.024 = 8.57% Inter-assay Sample n Mean SD CV% Serum 1 10 0.123 = 7.32% Serum 2 10 0.087 = 8.2% Serum 3 10 0.037 = 10.57% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Salmonella typhi is the causative agent of typhoid fever a contagious infection of the intestines that affects the whole body. In developing countries, typhoid often occurs in epidemics. Most people in the United States get typhoid as a result of visiting another country where the food or water supply has been contaminated. Symptoms usually start 1 to 3 weeks after exposure to the bacteria. Symptoms include: high fever, headache, sore throat, vomiting, diarrhea, skin rash and weakness. The symptoms may take 2 weeks or more to go away. Typhoid is spread when a person drinks or eats food and water contaminated by human waste (stool or urine) containing Salmonella typhi bacteria. A person who no longer has symptoms may still transmit the bacteria as a carrier. Testing for immunoglobulin G (IgG), IgA, and IgM antilipopolysaccharide (LPS) of Salmonella typhi antibodies by ELISA showed that the levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups. The ELISA assay (ab285279, E4679) was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4679 Salmonella Typhi IgM ELISA Kit. E4679-100 is the same size as the 96 test size of ab285279.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 20X Wash buffer 1 x 25ml Calibrator 1 x 1ml Enzyme conjugate 1 x 12ml Microplate 1 unit Negative Control 1 x 1ml Positive Control 1 x 1ml Sample Diluent 2 x 25ml Stop Solution 1 x 12ml TMB Substrate 1 x 12ml
Datasheets and documents
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Datasheet download
References (0)
ab285279 has not yet been referenced specifically in any publications.