Human Estrogen Receptor beta ELISA Kit (ab285292)
Key features and details
- Sensitivity: 0.188 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Estrogen Receptor beta ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.188 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
This estrogen receptor-beta ELISA kit (ab285292, K4268) is a sandwich ELISA assay for the quantitative measurement of human estrogen receptor-beta in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human estrogen receptor-beta captured from the samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K4268 Estrogen Receptor beta (ERß) (Human) ELISA Kit. K4268-100 is the same size as the 96 test size of ab285292.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin-Detection antibody (Concentrated) 1 x 120µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 2 vials Micro ELISA Plate 1 unit Plate Sealer 5 units SABC dilution buffer 1 x 10ml Sample and Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
Tissue specificity
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ER BETA
- ER-beta
- Erb
see all -
Database links
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285292 has not yet been referenced specifically in any publications.