Mouse free cholesterol ELISA Kit (ab285294)
Key features and details
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Mouse free cholesterol ELISA Kit
See all Cholesterol kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Range
1.25 ng/ml - 80 ng/ml -
Assay time
0h 90m -
Assay duration
Multiple steps standard assay -
Product overview
Mouse free cholesterol ELISA kit ab285294 (previously called QuickDetect free cholesterol (fCHOL) ELISA Kit, K4438) is a sandwich ELISA assay for measuring the quantity of free Cholesterol in mouse serum, plasma, cell culture supernatants cell culture, tissue homogenates and other biological fluids. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K4438 QuickDetect™ free cholesterol (fCHOL) (Mouse) ELISA Kit. K4438-100 is the same size as the 96 test size of ab285294.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 96 tests 20X Wash buffer 1 x 20ml 1 x 20ml Chomogen Solution A 1 x 6ml 1 x 6ml Chomogen Solution B 1 x 6ml 1 x 6ml HRP-Conjugate Reagent 1 x 6ml 1 x 6ml Micro ELISA Plate 1 unit 1 unit Microplate Sealer 2 units 2 units Special diluent 1 x 6ml 1 x 6ml Standard (80 ng/ml) 1 x 0.6ml 1 x 0.6ml Standard diluent 1 x 6ml 1 x 6ml Stop Solution 1 x 6ml 1 x 6ml -
Research areas
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Relevance
Cholesterol is a waxy substance that occurs naturally in all parts of the body and that the body needs to function normally. It is present in cell walls or membranes everywhere in the body, including the brain, nerves, muscle, skin, liver, intestines, and heart. The body uses cholesterol to produce many hormones, vitamin D, and the bile acids that help to digest fat. It takes only a small amount of cholesterol in the blood to meet these needs. However, if you have too much cholesterol in your bloodstream, it can lead to atherosclerosis, a condition in which fat and cholesterol are deposited in the walls of the arteries in many parts of the body, including the coronary arteries feeding the heart. In time, narrowing of the coronary arteries by atherosclerosis can produce the signs and symptoms of heart disease, including angina and heart attack.
Images
Datasheets and documents
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Datasheet download
References (0)
ab285294 has not yet been referenced specifically in any publications.