Human Aspartate Aminotransferase ELISA Kit (ab285299)
Key features and details
- Sensitivity: 0.188 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Aspartate Aminotransferase ELISA Kit
See all Aspartate Aminotransferase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma, Other biological fluids, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.188 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
This Human Aspartate Aminotransferase ELISA Kit (ab285299, E4319) is a sandwich ELISA assay for the quantitative measurement of human AST in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human AST captured from the samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4319 Aspartate Aminotransferase (AST) (Human) ELISA Kit. E4319-100 is the same size as the 96 test size of ab285299.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 1 x 30ml Antibody dilution buffer 1 x 10ml Biotin/Detection antibody (Concentrated) 1 x 120µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl Lyophilized Standard 2 vials Micro ELISA Plate 1 unit Plate sealers 5 units SABC dilution buffer 1 x 10ml Sample and Standard Dilution Buffer 1 x 20ml Stop Solution 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Function
Plays a key role in amino acid metabolism. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all -
Database links
- Entrez Gene: 2805 Human
- Omim: 138180 Human
- SwissProt: P17174 Human
- Unigene: 500756 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285299 has not yet been referenced specifically in any publications.