Recombinant B. subtilis Oxalate decarboxylase protein (Active) (ab285800)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Enzyme activity assay
Description
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Product name
Recombinant B. subtilis Oxalate decarboxylase protein (Active) -
Biological activity
Specific activity with 100 mM oxalate is approximately 2 U/mg. The specific activity is calculated using Oxalate Decarboxylase Activity Assay Kit.
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Purity
>= 98 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Bacillus subtilis -
Actual molecular weight
46 kDa -
Amino acids
1 to 385 -
Tags
His tag N-Terminus
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Description
Recombinant B. subtilis Oxalate decarboxylase protein (Active)
Specifications
Our Abpromise guarantee covers the use of ab285800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Enzyme activity assay
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Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 7262 Oxalate Decarboxylase, Active Bacterial Recombinant. 7262-1000 is the same size as the 1 mg size of ab285800.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
pH: 8.00
Constituents: 0.242% Tris, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE (4-20%) of ab285800: ab285800 was loaded under reducing conditions and stained with Coomassie
Blue. The protein showed a band at ~46 kDa.Lane 1: MW Markers
Lane 2: OxdC (2 µg)
Lane 3: OxdC (4 µg)
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Enzymatic Activity of ab285800: The enzymatic activity was calculated using a Oxalate Decarboxylase Activity Assay Kit (Colorimetric)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285800 has not yet been referenced specifically in any publications.