Lyophilized Exosome Standard (100 µg, Human Serum) (ab286846)
Overview
-
Product name
Lyophilized Exosome Standard (100 µg, Human Serum) -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called M1043 ExoStd™ Lyophilized Exosome Standard (100 µg, Human Serum, 2 vials). M1043-2 is the same size as the 2 vial size of ab286846.
This Lyophilized Exosome Standard (100 µg, Human Serum) ab286846 was previously known as ExoStd Lyophilized Exosome Standard (100 µg, Human Serum) M1043-2.
These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4ºC. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4°C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4ºC.
Properties
-
Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Concentration information loading...
Images
-
Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.
Western Blot comparison of exosomal markers on fresh (F), frozen (-20C) and lyophilized exosomes (L).
-
Lyophilization is the ideal method for preserving exosome stability. Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.
Comparison of exosomal markers on fresh and lyophilized on fresh (F), frozen (-20C) and lyophilized exosomes (L).
-
Comparative Nanosight analysis of freshly purified (lower panel) and lyophilized plasma exosomes (upper panel).
-
Comparative detection of CD81 on exosomes. 1- Fresh exosomes; 2- Frozen exosomes (-20°C); 3- Lyophilized exosomes.
Datasheets and documents
-
Datasheet download
References (0)
ab286846 has not yet been referenced specifically in any publications.