Bovine Serum Albumin - Cohn Fraction V (ab286863)
Overview
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Product name
Bovine Serum Albumin - Cohn Fraction V -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 7905 Bovine Serum Albumin – Cohn Fraction V, pH – 7.0. 7905-25 is the same size as the 25 g size of ab286863.
BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids the stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.
PURITY: ≥ 98% by SDS-PAGE gel
MOL. WEIGHT: ~ 66.43 kDa
ENDOTOXIN LEVEL: > 0.3 EU/mg by Gel Clot
FORM: Light Brown powder
OTHER PARAMETERS:
pH (1% solution): 6.6 – 7.0 by pH meter analysis
Calcium Content: < 2 mg/dL by Aesenazo III analysis
Chloride Content: < 25 mEq/L by ISE analysis
Potassium Content: < 0.2 mEq/L by ISE analysis
Sodium Content: < 20 mEq/L by ISE analysis
Moisture Content: 2.1 % by thermogravimetric analysis
Microbial Content: Passed lower limit. Growth monitored on a plate at 30-35°C for 48 hrs.
Mycoplasma content: Negative by E.I.A. Qualitative
Viral Agents (9 CFR 113): None Detected.
SOLUBILITY: 50 mg/ml in water, with 15 min of manual stirring.
Properties
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Form
Powder -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. It is important to note that this product is reported to be light sensitive. Store In the Dark. -
Storage buffer
pH: 6.60 -
Concentration information loading...
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab286863 has not yet been referenced specifically in any publications.