Reactive Oxygen Species (ROS) Detection Assay Kit (ab287839)
Overview
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Product name
Reactive Oxygen Species (ROS) Detection Assay Kit -
Detection method
Flow cytometry-fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased levels under oxidative stress, results in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. The ROS Detection Assay Kit ab287839 is designed for the detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as control for the measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy.
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Tested applications
Suitable for: FM, Functional Studiesmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 250 tests ROS Assay Buffer I 1 x 25ml 1 x 65ml 250X ROS Inducer 1 x 20µl 1 x 20µl 1000X ROS Label I 1 x 10µl 1 x 25µl
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab287839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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FM |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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FM
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Images
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Live cells labeled and treated with ROS Inducer and 100 µM of H2O2 according to the kit protocol.
2 x104 Jurkat cells per condition were analyzed by FACS. Plotted intensity values show a significant increase in ROS production between treatments and controls
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Live cells labeled and treated with ROS Inducer and 100µM of H2O2 according to the kit protocol.
1x105 Jurkat cells were analyzed on a plate reader. Mean+/- standard deviation plotted for 3 replicates per condition
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Profiling of ROS formation by Fluorescence Microscopy in Jurkat and MCF-7 live cells.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287839 has not yet been referenced specifically in any publications.