Highly Stable ATP Assay Kit (ab287863)
Overview
-
Product name
Highly Stable ATP Assay Kit
See all ATP kits -
Detection method
Luminescent -
Sample type
Tissue -
Assay type
Enzyme activity -
Sensitivity
> 1 nM -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals -
Product overview
In this kit, Highly Stable ATP Assay Kit ab287863 (previously known as StayBrite Higly stable ATP Bioluminescence assay kit, K791), we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China.
We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10??RLU/mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K791 StayBrite™ Highly Stable ATP Bioluminescence Assay Kit. K791-100 is the same size as the 100 test size of ab287863.
-
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1000 tests 10X StayBite Reaction buffer 1 x 20ml ATP Standard (MW 551) (Lyophilized) 1 vial StayBite Enzyme Mix (Lyophilized) 1 vial StayBite reconstitution buffer 1 x 11ml -
Research areas
-
Alternative names
- Adenosine 5' triphosphate
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab287863 has not yet been referenced specifically in any publications.