Recombinant Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337]
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies -
Description
Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
Host species
Rabbit -
Specificity
The antibody detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204 and ERK2 phosphorylated on Threonine 185 and Tyrosine 187. -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). The exact sequence is proprietary.
(Peptide available asab205613) -
Positive control
- WB: Serum starved A431 cell lysate treated with EGF. IHC-P: Human thyroid gland cancer tissue. ICC/IF: A431 cells +- EGF.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E337 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42 , 44 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200 - 1/250.
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Notes |
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WB
1/500 - 1/10000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42 , 44 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/250. |
Images
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Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) (ab32538) at dilution of 1:50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of 4% paraformaldehyde A431+-EGF(100ng/ml,5min) labelling Erk1 (pT202/pY204) + Erk2 (pT185/pY187) with ab32538 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 Goat-Anti-Rabbit IgG (ab150077) at dilution of 1/400. The counter stain was done with DAPI (blue).
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Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/500 dilution
Lane 2 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/2000 dilution
Lane 3 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/10000 dilution
All lanes : A431 treated with EGF for 10 minutes
Lysates/proteins at 0.1 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 , 44 kDa
Observed band size: 42.44 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesFirst-antibody diluted with 1% BSA.
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THP1 cells were incubated at 37°C for 3 minutes with vehicle control (0 μM) and different concentrations of ZK 756326 (ab120814). Increased expression of ERK1 (phospho T202 + Y204 ) + ERK2 (phospho T185 + Y187) (ab32538) in THP1 cells correlates with an increase in ZK 756326 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32538 at 1/500 dilution and ab17942 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Overlay histogram showing HeLa cells stained with ab32538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32538, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab32538 has been referenced in 29 publications.
- Zoi I et al. Polycystin-1 and hydrostatic pressure are implicated in glioblastoma pathogenesis in vitro. J Cell Mol Med 26:1699-1709 (2022). PubMed: 35106909
- Peng M et al. Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma. Genes Nutr 17:3 (2022). PubMed: 35164673
- Bruno C et al. Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes. Sci Rep 12:6268 (2022). PubMed: 35428750
- Cimmino F et al. FGFR1 is a potential therapeutic target in neuroblastoma. Cancer Cell Int 22:174 (2022). PubMed: 35488346
- Deng Y et al. ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma Progression. Front Pharmacol 13:858901 (2022). PubMed: 35600879