Anti-Nicastrin antibody (ab3444)
Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nicastrin antibody
See all Nicastrin primary antibodies -
Description
Rabbit polyclonal to Nicastrin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Nicastrin aa 650-750.
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Positive control
- WB: T98G, RSC96, mouse neuronal cells, HeLa, IMR-32, NIH/3T3, L6, mouse brain, rat brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the nicastrin family. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 23385 Human
- Entrez Gene: 59287 Mouse
- Entrez Gene: 289231 Rat
- Omim: 605254 Human
- SwissProt: Q92542 Human
- SwissProt: P57716 Mouse
- SwissProt: Q8CGU6 Rat
- Unigene: 517249 Human
see all -
Alternative names
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
see all
Images
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All lanes : Anti-Nicastrin antibody (ab3444) at 1 µg/ml
Lane 1 : HeLa whole cell extract
Lane 2 : IMR-32 whole cell extract (human neuroblastoma cell line)
Lane 3 : NIH/3T3 whole cell extract (mouse embryo fibroblast cell line)
Lane 4 : L6 whole cell extract (Rat skeletal muscle cell line)
Lane 5 : mouse brain
Lane 6 : rat brain
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa -
All lanes : Anti-Nicastrin antibody (ab3444) at 2 µg/ml
Lane 1 : T98G membrane extract (Human glioblastoma cell line)
Lane 2 : RSC96 membrane extract (spontaneously immortalized rat Schwann cell line )
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa -
Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab3444 has been referenced in 4 publications.
- Liu Y et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
- Pintchovski SA et al. Evidence that enzyme processivity mediates differential Aß production by PS1 and PS2. Curr Alzheimer Res 10:4-10 (2013). WB . PubMed: 22950863
- Steuble M et al. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport. Biol Open 1:761-74 (2012). PubMed: 23213470
- Massey LK et al. Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin. Biochem J 391:513-25 (2005). PubMed: 15975090