Recombinant Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Glutamate receptor 3/GluA3 antibody [EP813Y]
See all Glutamate receptor 3/GluA3 primary antibodies -
Description
Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
Database link: P42263 -
Positive control
- WB: Human brain tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP813Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
|
Notes |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa). |
Target
-
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Involvement in disease
Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2892 Human
- Entrez Gene: 53623 Mouse
- Entrez Gene: 29628 Rat
- Omim: 305915 Human
- SwissProt: P42263 Human
- SwissProt: Q9Z2W9 Mouse
- SwissProt: P19492 Rat
- Unigene: 377070 Human
see all -
Alternative names
- AMPA 3 antibody
- AMPA selective glutamate receptor 3 antibody
- AMPA-selective glutamate receptor 3 antibody
see all
Images
-
Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution (purified) + Human hippocampus lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 99 kDaBlocking and diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution (purified)
Lane 1 : Mouse hippocampus lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
-
Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + human brain lysate at 10 µg
Predicted band size: 99 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (19)
ab40845 has been referenced in 19 publications.
- Shao S et al. N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress. Neuroendocrinology 112:493-509 (2022). PubMed: 34348318
- Giesen J et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209
- Buss EW et al. Cognitive aging is associated with redistribution of synaptic weights in the hippocampus. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33593893
- Wright AL et al. Riluzole does not ameliorate disease caused by cytoplasmic TDP-43 in a mouse model of amyotrophic lateral sclerosis. Eur J Neurosci 54:6237-6255 (2021). PubMed: 34390052
- Cai Y et al. circ-NOL10 regulated by MTDH/CASC3 inhibits breast cancer progression and metastasis via multiple miRNAs and PDCD4. Mol Ther Nucleic Acids 26:773-786 (2021). PubMed: 34729247