Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)
Key features and details
- Mouse monoclonal [DE1] to Dengue Virus E glycoprotein
- Suitable for: ICC/IF, ELISA, Dot blot
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
- Isotype: IgG2b
Overview
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Product name
Anti-Dengue Virus E glycoprotein antibody [DE1]
See all Dengue Virus E glycoprotein primary antibodies -
Description
Mouse monoclonal [DE1] to Dengue Virus E glycoprotein -
Host species
Mouse -
Specificity
This antibody reacts with a conformational epitope on the envelope gylcoprotein of Dengue Virus 1, 2, 3 and 4. -
Tested applications
Suitable for: ICC/IF, ELISA, Dot blotmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 -
Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
DE1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/5 - 1/20.
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ELISA |
Use at an assay dependent concentration.
This antibody has been used in ELISA for the detection of degue IgM in patient serum samples. |
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/5 - 1/20. |
ELISA
Use at an assay dependent concentration. This antibody has been used in ELISA for the detection of degue IgM in patient serum samples. |
Dot blot
Use at an assay dependent concentration. |
Target
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Relevance
Dengue virus E glycoprotein is thought to be essential for membrane fusion and is also involved in binding to cellular receptors. It is the primary antigen responsible for inducing the protective immune response. -
Database links
- NCBI: ACA48835 Dengue virus 2
- SwissProt: P29990 Dengue virus 2
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Alternative names
- Dengue E glycoprotein antibody
- Dengue envelope glycoprotein antibody
- Dengue glycoprotein E antibody
see all
Images
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Dengue Virus 2 infected Vero cells stained with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.
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Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab41349 has been referenced in 8 publications.
- Prochnow H et al. Labyrinthopeptins Exert Broad-Spectrum Antiviral Activity through Lipid-Binding-Mediated Virolysis. J Virol 94:N/A (2020). PubMed: 31666384
- Anupriya MG et al. Sphingolipid signaling modulates trans-endothelial cell permeability in dengue virus infected HMEC-1 cells. Prostaglandins Other Lipid Mediat 136:44-54 (2018). ICC/IF . PubMed: 29733947
- Brai A et al. Human DDX3 protein is a valuable target to develop broad spectrum antiviral agents. Proc Natl Acad Sci U S A 113:5388-93 (2016). PubMed: 27118832
- Al-Alimi AA et al. Dengue virus type 2 (DENV2)-induced oxidative responses in monocytes from glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD normal subjects. PLoS Negl Trop Dis 8:e2711 (2014). Flow Cyt ; Dengue virus 2 . PubMed: 24625456
- Burlaud-Gaillard J et al. Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles. PLoS One 9:e93573 (2014). Electron Microscopy ; Dengue virus 2 . PubMed: 24681578
- Rothan HA et al. Identification of natural antimicrobial agents to treat dengue infection: In vitro analysis of latarcin peptide activity against dengue virus. BMC Microbiol 14:140 (2014). PubMed: 24885331
- Cardosa MJ et al. A nitrocellulose membrane based IgM capture enzyme immunoassay for etiological diagnosis of dengue virus infections. Clin Diagn Virol 3:343-50 (1995). PubMed: 15566815
- Cardosa MJ et al. IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens. Southeast Asian J Trop Med Public Health 23:726-9 (1992). PubMed: 1298081