Recombinant Anti-hnRNP K antibody [EP943Y] (ab52600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP943Y] to hnRNP K
- Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP K antibody [EP943Y]
See all hnRNP K primary antibodies -
Description
Rabbit monoclonal [EP943Y] to hnRNP K -
Host species
Rabbit -
Specificity
This antibody detects both phosphorylated and unphosphorylated hnRNP K. -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human hnRNP K (N terminal). The exact sequence is proprietary.
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Positive control
- WB:Jurkat cell lysate; ICC/IF: HeLa and MCF7 cells; Flow Cyt (intra): HeLa cells; IHC-P: Human breast carcinoma, Human lung carcinoma, Mouse stomach, and Rat stomach tissues; IP: Jurkat
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP943Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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IP |
1/20.
For unpurified use at 1/50 |
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IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250. |
|
Flow Cyt (Intra) |
1/20.
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Notes |
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WB
1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
IP
1/20. For unpurified use at 1/50 |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
Flow Cyt (Intra)
1/20. |
Target
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Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 117282 Rat
- Omim: 600712 Human
- SwissProt: P61978 Human
- SwissProt: P61979 Mouse
- SwissProt: P61980 Rat
- Unigene: 522257 Human
see all -
Alternative names
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/10000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab52600 (purified) at 1:20 dilution (2µg) immunoprecipitating hnRNP K in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52600 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52600 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1:50 dilution (1.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: ~60 kDa why is the actual band size different from the predicted? -
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
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Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
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ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab52600 has been referenced in 28 publications.
- Tan Y et al. A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis. Cell Death Dis 13:401 (2022). PubMed: 35461306
- Halder LD et al. Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth. mBio 13:e0356321 (2021). PubMed: 35132877
- Ji L et al. LINC01413/hnRNP-K/ZEB1 Axis Accelerates Cell Proliferation and EMT in Colorectal Cancer via Inducing YAP1/TAZ1 Translocation. Mol Ther Nucleic Acids 19:546-561 (2020). PubMed: 31927328
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Liu H et al. Involvement of the Wnt/ß-Catenin signaling pathway in the heterogenous nuclear ribonucleoprotein K-driven inhibition of proliferation and migration in head and neck squamous cell carcinoma. Oncol Lett 20:394 (2020). PubMed: 33193854