Anti-AAK1 antibody (ab59740)
Key features and details
- Rabbit polyclonal to AAK1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-AAK1 antibody
See all AAK1 primary antibodies -
Description
Rabbit polyclonal to AAK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide surrounding amino acid 850 of human AAK1.
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Positive control
- Mouse tissue lysate and rat kidney lysate.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3810 AAK1 Antibody. 3810-100 is the same size as the 100 µg size of ab59740.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa). |
Target
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Function
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 269774 Mouse
- Entrez Gene: 500244 Rat
- SwissProt: Q3UHJ0 Mouse
- SwissProt: P0C1X8 Rat
- Unigene: 221038 Mouse
- Unigene: 203307 Rat
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Alternative names
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
see all
Images
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All lanes : Anti-AAK1 antibody (ab59740) at 0.5 µg/ml
Lane 1 : Mouse tissue lysate
Lane 2 : Rat kidney tissue lysate
Predicted band size: 94 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab59740 has been referenced in 1 publication.
- Hu J et al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009). PubMed: 19412185