Recombinant human PKC epsilon protein (ab60847)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
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Product name
Recombinant human PKC epsilon protein
See all PKC epsilon proteins and peptides -
Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 1.74% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- KPCE_HUMAN
- MGC125656
- MGC125657
see all -
Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. - Information by UniProt
Images
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The specific activity of PKC epsilon (ab60847) was determined to be 1122 nmol/min/mg as per activity assay protocol and was equivalent to 1398 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab60847
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SDS PAGE analysis of ab60847
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Sample Kinase Activity Plot.
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ab60847 on SDS-PAGE, MW ~110 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab60847 has not yet been referenced specifically in any publications.