Anti-MYL9 antibody (ab64161)
Key features and details
- Rabbit polyclonal to MYL9
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MYL9 antibody
See all MYL9 primary antibodies -
Description
Rabbit polyclonal to MYL9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Bird, Mammals -
Immunogen
Synthetic peptide:
KKRPQRATSNVFAMFD
, corresponding to amino acids 12-27 of Human MYL9 -
Positive control
- IHC-P: Human Smooth Muscle (colon).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
Tissue specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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Database links
- Entrez Gene: 10398 Human
- Omim: 609905 Human
- SwissProt: P24844 Human
- Unigene: 504687 Human
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Alternative names
- 20 kDa myosin light chain antibody
- Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
- LC20 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab64161 has been referenced in 4 publications.
- Zhu K et al. Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p. Clin Res Hepatol Gastroenterol N/A:N/A (2019). PubMed: 31255531
- Chao CM et al. Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia. Hum Mol Genet 28:1429-1444 (2019). PubMed: 30566624
- Xie X & Percipalle P Elevated transforming growth factor ß signaling activation in ß-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features. J Cell Physiol N/A:N/A (2018). PubMed: 29851084
- Hudson CA et al. Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1). Mol Hum Reprod 18:265-79 (2012). WB . PubMed: 22155728