Anti-Ctip2 antibody (ab70453)
Key features and details
- Rabbit polyclonal to Ctip2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ctip2 antibody
See all Ctip2 primary antibodies -
Description
Rabbit polyclonal to Ctip2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).
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Positive control
- WB: Jurkat, MOLT-4 cells; IP:Jurkat; IHC: human prostate carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- Unigene: 709690 Human
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Lane 4 : MOLT-4 whole cell lysate at 50 µg
Lane 5 : MOLT-4 whole cell lysate at 15 µg
Predicted band size: 100 kDa
Exposure time: 3 minutesPrepared using NETN lysis
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Ctip2 was immunoprecipitated from Jurkat whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70453 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70453 at 1 µg/mL.
Lane 1: rabbit anti-Bcl11b antibody IP in Jurkat whole cell lysate.
Lane 2: ab70453 IP in Jurkat whole cell lysate.
Lane 3: Control IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab70453 has been referenced in 5 publications.
- Sanders B et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
- Marcilla D et al. (Immuno)histological Analysis of Ewing Sarcoma. Methods Mol Biol 2226:49-64 (2021). PubMed: 33326093
- Orth MF et al. High Specificity of BCL11B and GLG1 for EWSR1-FLI1 and EWSR1-ERG Positive Ewing Sarcoma. Cancers (Basel) 12:N/A (2020). PubMed: 32164354
- Forbes LH & Andrews MR Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract. Front Cell Neurosci 13:26 (2019). PubMed: 30809126
- Baldauf MC et al. Robust diagnosis of Ewing sarcoma by immunohistochemical detection of super-enhancer-driven EWSR1-ETS targets. Oncotarget 9:1587-1601 (2018). PubMed: 29416716