Recombinant human Interferon beta protein (Active) (ab71475)
Key features and details
- Expression system: CHO cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
Description
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Product name
Recombinant human Interferon beta protein (Active)
See all Interferon beta proteins and peptides -
Biological activity
Determined by a cytotoxicity assay using human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Purity
>= 95 % SDS-PAGE.
>= 95% HPLC analyses. Due to glycosylation, IFN-ß has an approximate MW of 22.3 kDa based on SDS-PAGE gel -
Endotoxin level
< 1.000 Eu/µg -
Expression system
CHO cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN -
Predicted molecular weight
20 kDa -
Amino acids
22 to 187 -
Additional sequence information
Full length mature protein, minus the signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab71475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
HPLC
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- beta-interferon
- Fibroblast interferon
- IFB
see all -
Function
Has antiviral, antibacterial and anticancer activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab71475 has been referenced in 3 publications.
- Constantin D et al. APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites. FEBS J 289:808-831 (2022). PubMed: 34528388
- Lyu X et al. Preventing SARS-CoV-2 Infection Using Anti-spike Nanobody-IFN-β Conjugated Exosomes. Pharm Res N/A:1-9 (2022). PubMed: 36163411
- Tsai M et al. The E3 ligase subunit FBXO45 binds the interferon-λ receptor and promotes its degradation during influenza virus infection. J Biol Chem 298:102698 (2022). PubMed: 36379255