Recombinant Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies -
Description
Rabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa whole cell lysate (ab150035), untreated or treated with calyculin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2847Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Tissue specificity
Expressed in many tissues, highest levels in skeletal muscle. -
Involvement in disease
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
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Database links
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
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Alternative names
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
Images
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All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820) at 1/2000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab75820 has not yet been referenced specifically in any publications.